Plasmid Preparation:Article Title: Selective advantage of epigenetically disrupted cancer cells via phenotypic inertia.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgRNA sequences in the engineered pLenti-BSD-sgRNA plasmid used for 10x Gemonics scRNAseq with Feature Barcoding Technology for CRISPR screening This paper Table S7 Synthetic crRNAs used to generate KO cells This paper Table S7 Recombinant DNA Plasmid: pCW-Cas9 Monserrat et al., 2021 N/A Plasmid: psPax2 Trono Lab Addgene; Cat#12260; RRID:Addgene_12260 Plasmid: pMD2.G Trono Lab Addgene; Cat#12259; RRID:Addgene_12259 Plasmid: pAdVAntage Vector Promega Cat#E1711 Plasmid: arrayed lentiviral sgRNA library Henser-Brownhill et al., 2017 N/A Plasmid: pTRIP-SFFV-EGFP-NLS Raab et al., 2016 Addgene; Cat#86677; RRID:Addgene_86677 Plasmid: pUAS-mCherry-NLS Zhang et al., 2017 Addgene; Cat#87695; RRID:Addgene_87695 Plasmid: pTRIP-SFFV-mCherry-NLS This paper N/A Plasmid: PiggyBac transposon carrying FRET biosensor Kondo et al., 2021 N/A Plasmid: PB transposase (PBase) Liang et al., 2009 N/A Plasmid: pLenti-BSD-sgRNACapture_seq_1E This paper Table S7 Software and algorithms ImageJ 1.45s NIH RRID:SCR_003070; https://imagej.nih. gov/ij/ QuPath-0.2.2 Bankhead, P. et al., 2017 RRID:SCR_018257; https://qupath. github.io Zeiss Zen software (v2.3) ZEISS N/A Incucyte S3 Live-Cell Analysis System Sartorius RRID:SCR_019874; https://www.sartorius. com/en/products/live-cell-imaging- analysis/live-cell-analysis-instruments Gene Set Enrichment Analysis (GSEA) GSEA (Broad Institute) RRID:SCR_003199; http://www. broadinstitute.org/gsea/ Prism 9 GraphPad Software RRID:SCR_002798; https://www. graphpad.com/ CellRanger (v3.0.2) 10x Genomics RRID:SCR_017344; https://support.
CRISPR:Article Title: Selective advantage of epigenetically disrupted cancer cells via phenotypic inertia.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgRNA sequences in the engineered pLenti-BSD-sgRNA plasmid used for 10x Gemonics scRNAseq with Feature Barcoding Technology for CRISPR screening This paper Table S7 Synthetic crRNAs used to generate KO cells This paper Table S7 Recombinant DNA Plasmid: pCW-Cas9 Monserrat et al., 2021 N/A Plasmid: psPax2 Trono Lab Addgene; Cat#12260; RRID:Addgene_12260 Plasmid: pMD2.G Trono Lab Addgene; Cat#12259; RRID:Addgene_12259 Plasmid: pAdVAntage Vector Promega Cat#E1711 Plasmid: arrayed lentiviral sgRNA library Henser-Brownhill et al., 2017 N/A Plasmid: pTRIP-SFFV-EGFP-NLS Raab et al., 2016 Addgene; Cat#86677; RRID:Addgene_86677 Plasmid: pUAS-mCherry-NLS Zhang et al., 2017 Addgene; Cat#87695; RRID:Addgene_87695 Plasmid: pTRIP-SFFV-mCherry-NLS This paper N/A Plasmid: PiggyBac transposon carrying FRET biosensor Kondo et al., 2021 N/A Plasmid: PB transposase (PBase) Liang et al., 2009 N/A Plasmid: pLenti-BSD-sgRNACapture_seq_1E This paper Table S7 Software and algorithms ImageJ 1.45s NIH RRID:SCR_003070; https://imagej.nih. gov/ij/ QuPath-0.2.2 Bankhead, P. et al., 2017 RRID:SCR_018257; https://qupath. github.io Zeiss Zen software (v2.3) ZEISS N/A Incucyte S3 Live-Cell Analysis System Sartorius RRID:SCR_019874; https://www.sartorius. com/en/products/live-cell-imaging- analysis/live-cell-analysis-instruments Gene Set Enrichment Analysis (GSEA) GSEA (Broad Institute) RRID:SCR_003199; http://www. broadinstitute.org/gsea/ Prism 9 GraphPad Software RRID:SCR_002798; https://www. graphpad.com/ CellRanger (v3.0.2) 10x Genomics RRID:SCR_017344; https://support.
Recombinant:Article Title: Selective advantage of epigenetically disrupted cancer cells via phenotypic inertia.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgRNA sequences in the engineered pLenti-BSD-sgRNA plasmid used for 10x Gemonics scRNAseq with Feature Barcoding Technology for CRISPR screening This paper Table S7 Synthetic crRNAs used to generate KO cells This paper Table S7 Recombinant DNA Plasmid: pCW-Cas9 Monserrat et al., 2021 N/A Plasmid: psPax2 Trono Lab Addgene; Cat#12260; RRID:Addgene_12260 Plasmid: pMD2.G Trono Lab Addgene; Cat#12259; RRID:Addgene_12259 Plasmid: pAdVAntage Vector Promega Cat#E1711 Plasmid: arrayed lentiviral sgRNA library Henser-Brownhill et al., 2017 N/A Plasmid: pTRIP-SFFV-EGFP-NLS Raab et al., 2016 Addgene; Cat#86677; RRID:Addgene_86677 Plasmid: pUAS-mCherry-NLS Zhang et al., 2017 Addgene; Cat#87695; RRID:Addgene_87695 Plasmid: pTRIP-SFFV-mCherry-NLS This paper N/A Plasmid: PiggyBac transposon carrying FRET biosensor Kondo et al., 2021 N/A Plasmid: PB transposase (PBase) Liang et al., 2009 N/A Plasmid: pLenti-BSD-sgRNACapture_seq_1E This paper Table S7 Software and algorithms ImageJ 1.45s NIH RRID:SCR_003070; https://imagej.nih. gov/ij/ QuPath-0.2.2 Bankhead, P. et al., 2017 RRID:SCR_018257; https://qupath. github.io Zeiss Zen software (v2.3) ZEISS N/A Incucyte S3 Live-Cell Analysis System Sartorius RRID:SCR_019874; https://www.sartorius. com/en/products/live-cell-imaging- analysis/live-cell-analysis-instruments Gene Set Enrichment Analysis (GSEA) GSEA (Broad Institute) RRID:SCR_003199; http://www. broadinstitute.org/gsea/ Prism 9 GraphPad Software RRID:SCR_002798; https://www. graphpad.com/ CellRanger (v3.0.2) 10x Genomics RRID:SCR_017344; https://support.
Software:Article Title: Selective advantage of epigenetically disrupted cancer cells via phenotypic inertia.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgRNA sequences in the engineered pLenti-BSD-sgRNA plasmid used for 10x Gemonics scRNAseq with Feature Barcoding Technology for CRISPR screening This paper Table S7 Synthetic crRNAs used to generate KO cells This paper Table S7 Recombinant DNA Plasmid: pCW-Cas9 Monserrat et al., 2021 N/A Plasmid: psPax2 Trono Lab Addgene; Cat#12260; RRID:Addgene_12260 Plasmid: pMD2.G Trono Lab Addgene; Cat#12259; RRID:Addgene_12259 Plasmid: pAdVAntage Vector Promega Cat#E1711 Plasmid: arrayed lentiviral sgRNA library Henser-Brownhill et al., 2017 N/A Plasmid: pTRIP-SFFV-EGFP-NLS Raab et al., 2016 Addgene; Cat#86677; RRID:Addgene_86677 Plasmid: pUAS-mCherry-NLS Zhang et al., 2017 Addgene; Cat#87695; RRID:Addgene_87695 Plasmid: pTRIP-SFFV-mCherry-NLS This paper N/A Plasmid: PiggyBac transposon carrying FRET biosensor Kondo et al., 2021 N/A Plasmid: PB transposase (PBase) Liang et al., 2009 N/A Plasmid: pLenti-BSD-sgRNACapture_seq_1E This paper Table S7 Software and algorithms ImageJ 1.45s NIH RRID:SCR_003070; https://imagej.nih. gov/ij/ QuPath-0.2.2 Bankhead, P. et al., 2017 RRID:SCR_018257; https://qupath. github.io Zeiss Zen software (v2.3) ZEISS N/A Incucyte S3 Live-Cell Analysis System Sartorius RRID:SCR_019874; https://www.sartorius. com/en/products/live-cell-imaging- analysis/live-cell-analysis-instruments Gene Set Enrichment Analysis (GSEA) GSEA (Broad Institute) RRID:SCR_003199; http://www. broadinstitute.org/gsea/ Prism 9 GraphPad Software RRID:SCR_002798; https://www. graphpad.com/ CellRanger (v3.0.2) 10x Genomics RRID:SCR_017344; https://support.
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